Alkyl iodides react at a faster rate than alkyl fluorides due to the weak C-I bond. Iodo alkanes participate in a variety of organic synthesis reactions, which include the Simmons-Smith reaction (cyclopropanation using iodomethane), 144-48-9, formula is C2H4INO, Name is 2-Iodoacetamide. Williamson ether synthesis, Wittig reaction, Grignard reaction, alkyl coupling reactions, and Wurtz reaction. Quality Control of 144-48-9.
Tavares, Lethicia Souza;Ralph, Maria Taciana;Batista, Jacqueline Ellen Camelo;Sales, Ana Clarissa;Ferreira, Laisla Carolina Andrade;Usman, Usman Abdulhadi;da Silva, Valdemiro Amaro Junior;Ramos, Marcio Viana;Lima-Filho, Jose Vitor research published 《 Perspectives for the use of latex peptidases from Calotropis procera for control of inflammation derived from Salmonella infections》, the research content is summarized as follows. Anti-inflammatory properties have been attributed to latex proteins of the medicinal plant Calotropis procera. A mixture of cysteine peptidases (LPp2) from C. procera latex was investigated for control of inflammatory mediators and inflammation in a mouse model of Salmonella infection. LPp2 peptidase activity was confirmed by the BANA assay. Cytotoxicity assays were conducted with immortalized macrophages. Peritoneal macrophages (pMO) from Swiss mice were stimulated with lipopolysaccharide (LPS) in 96-well plates and then cultured with nontoxic concentrations of LPp2. Swiss mice i.v. received LPp2 (10 mg/kg) and then were challenged i.p. with virulent Salmonella enterica Ser. Typhimurium. LPp2 was not toxic at dosages lower than 62.2μg/mL. LPp2 treatments of pMO stimulated with LPS impaired mRNA expression of pro-inflammatory cytokines IL-1β, TNF-α IL-6 and IL-10. LPp2 increased the intracellular bacterial killing in infected pMO. Mice given LPp2 had a lower number of leukocytes in the peritoneal cavity in comparison to control groups 6 h after infection. The bacterial burden and histol. damage were widespread in target organs of mice receiving LPp2. We conclude that LPp2 contains peptidases with strong anti-inflammatory properties, which may render mice more susceptible to early disseminated infection caused by Salmonella.
144-48-9, 2-Iodoacetamide is a synthetic retinoid that binds to the DNA of cells, altering transcription. It also has been found to be effective in treating bowel disease and has been shown to have dna binding activity. The compound was synthesized by attaching iodine molecules to acetamide. 2-Iodoacetamide targets the protein thiols on the surface of cells, which are responsible for oxidation and damage due to reactive oxygen species (ROS). This compound is metabolized by alcohol dehydrogenase and can be used as a biological sample or natural compound is a compound used as an electrophile for covalent modification of nucleophilic residues on proteins (cysteine, methionine, histidine). When modifying the active-site residues of cysteine proteases, α-Iodoacetamide acts as an irreversible inhibitor of these enzymes.
2-Iodoacetamide used in peptide mapping because it covalently binds with thiols in cysteine residues, thereby preventing disulfide bond formation. By virtue of reaction with cysteine, it is an irreversible inhibitor of enzymes with cysteine at the active site. Also reacts with histidine residues though much more slowly, and this activity is responsible for inhibition of ribonuclease.
An alkylating sulfhydryl reagent. Its actions are similar to those of iodoacetate., Quality Control of 144-48-9
Referemce:
Iodide – Wikipedia,
Iodide – an overview | ScienceDirect Topics – ScienceDirect.com