The preparation of ester heterocycles mostly uses heteroatoms as nucleophilic sites, which are achieved by intramolecular substitution or addition reactions. Compound: 2-Methylglutaronitrile( cas:4553-62-2 ) is researched.Synthetic Route of C6H8N2.Fallon, R. D.; Stieglitz, B.; Turner, I. Jr. published the article 《A Pseudomonas putida capable of stereoselective hydrolysis of nitriles》 about this compound( cas:4553-62-2 ) in Applied Microbiology and Biotechnology. Keywords: stereoselective nitrile hydratase amide Pseudomonas; nitrilase stereoselective Pseudomonas. Let’s learn more about this compound (cas:4553-62-2).
P. putida NRRL-18668 contains a nitrile hydratase capable of stereoselective hydrolysis of 2-(4-chlorophenyl)-3-methylbutyronitrile at >90% enantiomeric excess (ee) to the (S)-amide. This soil isolate was recovered from enrichments using (R,S)-2-methylglutaronitrile as the sole nitrogen source. Enzyme expression is constitutive and does not show a high level of catabolite repression. The organism is capable of growth on a wide variety of aliphatic mono- and dinitrile compounds The hydrolysis activity on propionitrile is ∼10.3 μmol/h-mg wet cells. The enzyme in cell-free preparations is inhibited by a number of heavy metals, phenylhydrazine, and cyanide. Substrate specificity is broad, with highest rates shown on C4 and C5 aliphatic mononitriles. The strain appears somewhat unusual in its dependence on Co supplementation for maximum enzyme activity and the ability to hydrolyze some aromatic nitriles. This strain is also capable of a 2-step hydrolysis of 2-(4-isobutylphenyl)-propionitrile and 2-(6-methoxy-2-naphthyl)-propionitrile to the (S, with stereoselectivity residing primarily in the aliphatic amidase. This appears to be the 1st description of a stereoselective nitrile hydratase from a gram-neg. organism.
Compounds in my other articles are similar to this one(2-Methylglutaronitrile)Synthetic Route of C6H8N2, you can compare them to see their pros and cons in some ways,such as convenient, effective and so on.
Reference:
Iodide – Wikipedia,
Iodide – an overview | ScienceDirect Topics – ScienceDirect.com