Rana, Rahul; Chang, Qing; Bassan, Jay; Chow, Sue; Hedley, David; Nitz, Mark published their research in ChemBioChem in 2021. The article was titled 《An Iodinated DAPI-Based Reagent for Mass Cytometry》.Recommanded Product: 516-12-1 The article contains the following contents:
Multiparametric single-cell anal. has seen dramatic improvements with the introduction of mass cytometry (MC) and imaging mass cytometry (IMC). These technologies expanded the number of biomarkers that can be identified simultaneously by using heavy-isotope-tagged antibody reagents. Small-mol. probes bearing heavy isotopes are emerging as addnl. useful functional reporters of cellular features. Realizing this, we explored the iodination of DAPI to produce a heavy-atom-substituted derivative of the commonly used fluorescent DNA stain. Although exhibiting a drastically reduced fluorescence emission profile, I-DAPI retains strong binding affinity for DNA. I-DAPI was used to detect cellular DNA in MC and IMC assays with comparable efficiency to known Ir-containing DNA intercalators. This work suggests repurposing well-known colorimetric stains through simple reactions could be an effective strategy to develop new, functional MC and IMC reagents. After reading the article, we found that the author used 1-Iodopyrrolidine-2,5-dione(cas: 516-12-1Recommanded Product: 516-12-1)
1-Iodopyrrolidine-2,5-dione(cas: 516-12-1) is used in the preparation of vinyl sulfones from olefins and benzenesulfinic acid. It acts as a source for I+ and involved in Hunsdiecker reactions for the conversion of cinnamic acids, and propiolic acids to the corresponding alfa-halostyrenes and 1-halo-1-alkynes respectively. Recommanded Product: 516-12-1
Referemce:
Iodide – Wikipedia,
Iodide – an overview | ScienceDirect Topics – ScienceDirect.com