《Ethynylation of Cysteine Residues: From Peptides to Proteins in Vitro and in Living Cells》 was written by Tessier, Romain; Nandi, Raj Kumar; Dwyer, Brendan G.; Abegg, Daniel; Sornay, Charlotte; Ceballos, Javier; Erb, Stephane; Cianferani, Sarah; Wagner, Alain; Chaubet, Guilhem; Adibekian, Alexander; Waser, Jerome. Application In Synthesis of 2-Iodobenzoic acid And the article was included in Angewandte Chemie, International Edition in 2020. The article conveys some information:
Current approaches to introduce terminal alkynes for bioorthogonal reactions into biomols. still present limitations in terms of either reactivity, selectivity, or adduct stability. The authors present a method for the ethynylation of cysteine residues based on the use of ethynylbenziodoxolone (EBX) reagents. The acetylene group is directly introduced onto the thiol group of cysteine and can be used for copper-catalyzed alkyne-azide cycloaddition (CuAAC) without further processing. Labeling proceeded with reaction rates comparable to or higher than the most often used iodoacetamide on peptides or maleimide on the antibody trastuzumab, and high cysteine selectivity was observed The reagents were also used in living cells for cysteine proteomic profiling and displayed improved coverage of the cysteinome compared to previously reported iodoacetamide or hypervalent iodine reagents. Fine-tuning of the EBX reagents allows optimization of their reactivity and phys. properties. The experimental part of the paper was very detailed, including the reaction process of 2-Iodobenzoic acid(cas: 88-67-5Application In Synthesis of 2-Iodobenzoic acid)
2-Iodobenzoic acid(cas: 88-67-5) belongs to organic iodides. The carbon-iodine bond is weaker than other carbon-halogen bonds due to the poor electronegative nature of the iodine atom. Application In Synthesis of 2-Iodobenzoic acid Organic iodides can be alkyl, alkenyl, or alkynyl, and all of them are very reactive toward with many kinds of nucleophiles.
Referemce:
Iodide – Wikipedia,
Iodide – an overview | ScienceDirect Topics – ScienceDirect.com