Chemistry is an experimental science, Computed Properties of C7H5IO2, and the best way to enjoy it and learn about it is performing experiments.Introducing a new discovery about 619-58-9, Name is 4-Iodobenzoic acid, molecular formula is C7H5IO2, belongs to iodides-buliding-blocks compound. In a document, author is BHATTACHARYYA, DK.
IODIDE MODULATION OF THE EDTA-INDUCED IODINE REDUCTASE-ACTIVITY OF HORSERADISH-PEROXIDASE BY INTERACTION AT OR NEAR THE EDTA-BINDING SITE
Horseradish peroxidase (HRP) catalyses the reduction of iodinium ion (I+) to iodide by H2O2 in the presence of EDTA. I+ reduction occurs optimally at pH 6 whereas the enzyme catalyses iodide oxidation optimally at pH 3.5. Thus the two activities reside on the same enzyme with two characteristic pH optima. Iodide modulates the expression of the reductase activity by EDTA. Higher concentrations of iodide inhibit the reductase activity by EDTA. Nitrite, an electron donor, acts similarly to iodide. Both EDTA and nitrite competitively inhibit iodide oxidation, indicating that they compete with iodide for the same binding site for electron flow to the haem iron group. However, unlike iodide, EDTA converts compound I, not into the native enzyme. but into a compound absorbing at 416 nm which reduces I+ and then returns to the native form. The apparent equilibrium dissociation constant, K(D), for the formation of the EDTA-HRP complex (15 mM) is doubled in the presence of iodide, indicating interference with EDTA binding by iodide. EDTA binds away from the haem iron centre and not through intramolecular Ca2+. The pH-dependence of EDTA binding indicates that an ionizable group of the enzyme with pK(a) 5.8, presumably a distal histidine, controls the binding. The data suggest that iodide competes with EDTA for compound I and modulates the iodine reductase activity by limiting the formation of the 416 nm-absorbing active compound.
Sometimes chemists are able to propose two or more mechanisms that are consistent with the available data. If a proposed mechanism predicts the wrong experimental rate law, however, the mechanism must be incorrect.Welcome to check out more blogs about 619-58-9, in my other articles. Computed Properties of C7H5IO2.